Troubleshooting
In this section, we document commonly encountered issues.
Why does my prediction consistently fail?
Although we strive to catch validation issues upon submission, there are still circumstances under which predictions may fail with cryptic error messages. The most common issue is that the prediction generates a result set that is over the maximum threshold of 100 megabytes. To give an idea of the kind of input that can generate a result this large for our class I prediction tool, a 1500 residue sequence x 7 lengths x 27 alleles prediction results in roughly 100 megabytes of result output. Please try reducing the size of your inputs or number of parameter permutations to address these issues. Alternatively, you may download the tools to run on your own hardware to avoid size constraints.
What are the input size limits?
T Cell Class I currently has an input size limit of 1,000,000 characters.
PepX currently accepts up to 10,000 peptides.
PepMatch currently has an input size limit of 1,000 peptides.
Clustering currently has an input size limit of 1,000 peptides.
Peptide Variant Comparison currently has an input size limit of 5,000 pairs of peptides.
Mutated Peptide Generator currently accepts VCF files with size limits of up to 5,000 lines.
Why does PepMatch not seem to complete?
There have been cases where large lists of peptides submitted to PepMatch cause the results section to be in a constant ‘loading results’ state. To mitigate this, the user should provide an email address in the ‘Info’ section of the ‘Pipeline Map’ before running the PepMatch step. If the email address is supplied, the results are sent to email even in cases when they do not initially appear on the web page.
How do I get more personalized support?
For help with any topics related to the IEDB NG Tools, please contact us at help@iedb.org to create a ticket. Please provide as much detail as possible, including the full URL of the page/pipeline in question, so that our agents can effectively address your issues.